The thickness of the fixed aqueous layer of DOXIL® from Presome ACD-1 : 2.1 nm
1) K. Shimada, A. Miyagishima, Y. Sadzuka, Y. Nozawa, Y. Mochizuki, H. Ohshima and S. Hirota, Determination of the thickness of the fixed aqueous layer around polyethyleneglycol-coated liposomes. Journal of Drug Targeting, 1995, 3, 283
Negative staining: The samples were let to absorbed 400 mesh carbon coated grid and were put into 2% phospho tungstic acid solution (㏗7.0) for a few seconds.
Observation and imaging: The samples were observed by a transmission electron microscope (JEM-1200EX; JEOL Ltd.,) at an acceleration voltage of 80 kV. Digital images (2048x2048 pixels) were taken with a CCD camera (VELETA, Olympus Soft Imaging Solutions GmbH).